Cloning Machine Notebook
plant qty date started notes
Black Mulberry 15 3/18/2012 signs of callus at 7 days
Goumi 5 5 3/18/2012

+++Here are some notes about plant propagation using a cloning machine.

NAA more effective on rooting apple cuttings
REGENERATION OF APPLE PLANTS FROM SHOOT MERISTEM
http://site.iugaza.edu.ps/tbashiti/files/2010/02/Micrp3.pdf

From The propagation of apple trees on their own roots By Jacob Kingsley Shaw, Massachusetts Agricultural Experiment Station, 1919
PROPAGATION BY CUTTINGS There are few published records of attempts to propagate apple trees by cuttings Doubtless many have been made and not reported for the uniform results on record may be described in the single word failure Luke 1 attempted to root apple cuttings of various sizes and lengths at cutting bed temperatures of 64 and 67 All failed to strike root Luke was able to induce root cuttings to grow with fairly good success Attempts to root apple cuttings were made during the summer of 1912 Green wood cuttings 3 to 4 inches long were made in early August and September and set in sand in the greenhouse Powdered charcoal was also used as a propagating medium both alone and as a one half inch layer over sand with the hope that it might check disease Bottom heat in varying degrees was used in some cases also an enclosed propagating frame In short an effort was made to provide the best possible conditions for cuttings Something over a thousand cuttings of several different varieties were made The results were much the same in all cases The cuttings formed a callus varying somewhat with the variety and the buds started out until the leaves were about one fourth inch long This occupied about two weeks after which growth ceased The final result was the same in practically all cases Of the 1,000 or more cuttings only a single one of the Fall Pippin variety rooted and that only a single short shoot that was broken off in removing from the sand so that it failed to grow Fig 1 is a typical representation of the range of development of callus and leaf Arranged in order of callus development the varieties are Yellow Transparent Fall Pippin Red Astrachan Bough Sweet Ben Davis Wagener As will be shown later these varieties may be induced to root from the scion when grown by the nurse root method more or less readily according to the variety There is however little or no correlation between callus growth and root formation as may be seen by comparison with the numbers rooting shown in Table 2 One lot of cuttings was watered with a nutrient solution using a formula in common use for growing seedlings The only effect was a noticeable growth of green algm over the surface of the sand The cutting growth was hindered rather than helped In spite of these failures it is the opinion of the writer that it is possible to grow apple trees from cuttings To an inquiry addressed to many of the leading nurserymen of the country thirty five replied that they had never seen cuttings or prunings from the trees taking root while seventeen professed to have observed such an occurrence though none of them considered it at all common One nurseryman reported having planted well callused scions in a mixture of sand and soil and that the best stand we ever had was something less than 10 per cent of the cuttings planted The trees were weak for a year or two The late TV Munson of Denison Tex says I have often had apple and even peach switches cut from the trees in February and stuck into the ground very sandy for label sticks take root and grow off well In the spring of 1913 a considerable number of root cuttings from young trees were planted in the nursery row No record was kept of them but they made a good stand though growth was very slow the first season It is the practice of at least one nursery firm to dig trees aheady established on their own roots once in two years and cut off the roots for propagation by root cuttings The trees are then replanted and a new crop of roots grown In a later experience of the writer root cuttings from the root system of bearing trees were used in an attempt to propagate the stock variety This resulted in almost a complete failure The roots used were from one quarter to one half inch in diameter and when planted in the open about 3 inches long Others planted in the greenhouse were about 1 inches long Whether older roots propagate with greater difficulty or whether some unfavorable conditions not readily seen interfered with success cannot be told with certainty.
http://books.google.com/books?id=ZQInAQAAMAAJ&pg=PA75&img=1&zoom=3&hl=en&sig=ACfU3U164-BCEHh-1d2g9upCzoKlr4d-tQ&ci=41%2C141%2C859%2C1434&edge=0

+++What is a cloning machine?
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+++Procedure
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+++Success with individual plants
Rosa canina - Dog Rose
Eleaganus multiflora - Goumi
Lettuce Leaf Basil
Tomato

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