The following information is from some mushroom cloning experiments with 3% hydrogen peroxide. At first it was thought that spores had germinated in the peroxide. THen someone noted that peroxide kills spores, and a spore print scraped into peroxide failed to germinate. Someone suggested that the bubbling broke the mycelium into small pieces, and those started to grow. It almost works. Mold was a problem except for one guy that got lucky.
from https://mycotopia.net/forums/contamination-pests-bugs/16629-spore-germination-peroxide.html
This half pint jar appears to show many germinating spores in pure 3% peroxide after two weeks of soaking. The jar was simply washed and dried, then filled halfway with peroxide. A fresh whole mushroom was dropped in and just sat there for two weeks. After a few days i added a tiny amount of Karo straight from the bottle to encourage clone type growth. I was hoping to maybe see some mycelium eventually grow from the shroom itself once the peroxide had neutralized , i never expected to see spores germinate though. The shroom itself is still well preserved with no sign of any new growth. So i'm gonna inject this solution into some spawn jars and find out whats up. Hopefully any contam has been killed by the prolonged contact with the H2o2.
Just took a sample of solution and scoped it out 60x…..It's def mycelium. Under the scope there were thousands of tiny threads of mycelium.
Another guy used no karo just straight h2o2. if you look at the photo of the inner stem in the peroxide you can tell it was ready to GO. Like i said earlier, not very sterile conditions while i transfered the inner tissue.
Here's an update on the progress of that peroxidated mushroom that released it's spores, then germinated in solution. The growing spores were sucked up in a syringe and injected into three quart jars of shakable cake. After 6 days ,new growth is now visible in several spots in all jars, but appears that only a small fraction of the injected material survived transfer. No contamination is visible so far.
In the follow up test - Using a spore print in pure peroxide [3%] we are at day five. I'm thinking now, that Bobcat's idea maybe correct - in that tiny fragments of the shroom are broken off through the oxidizing proccess and growing out on the jar bottom…The spore only jar is now at day 20 with no sign of life.
I had an Agaricus tray which developed a patch of trich mold , so i took a small clump of still healthy myc and dropped in a peroxide jar for 8 hours , then transfered to a spawn jar for re-growing. 3 days later the whole jar is exploding with mycelium.. so I'm pretty stoked about that. The idea that u can save a contam'd tray by reverting back to spawn is pretty exciting to me because it's so easy ..Once again though - it's too early in the game to say it's def gonna work.
Also In the other experiment , dried frozen mush dropped in peroxide for just 8 hours ,then transfered to shakable cake spawn jars - is now showing numerous points of growth at day 3.
